The Scantibodies PTH AccuratioTM Panel

Scantibodies Clinical Laboratory (SCL) offers a panel which includes the values for both hormones produced by the parathyroid gland, as well as the critical 1-84 PTH/7-84 PTH ratio computed from tests run with highly sensitive and specific antibodies. The current technology in PTH testing measures only one of the PTH hormones - the 1-84 PTH - and is often referred to as the "Whole PTH Assay". Here are some important points of vital information which have recently been discovered and documented regarding PTH. It is good to consider these points when selecting a laboratory to do the PTH testing for your practice or clinic:

  1. The parathyroid glands make two parathyroid hormones: 1-84 PTH and 7-84 PTH. Data from more than 200 bone biopsies have revealed that:
    1. 1-84 PTH raises bone turnover
    2. 7-84 PTH lowers bone turnover
  2. High bone turnover disease and adynamic bone disease are conditions that are observed histologically, with bone biopsy being the "gold standard" for detecting and monitoring these conditions.
  3. An excess of 1-84 PTH compared to 7-84 PTH causes high bone turnover disease or hyperparathyroid bones (HPT).
  4. An excess of 7-84 PTH compared to 1-84 PTH causes adynamic bone disease.
  5. Adynamic bone disease is the most prevalent bone disease (i.e., it occurs more often than high bone turnover disease.)
  6. Calcification is associated with adynamic bone disease and not with high bone turnover disease.
  7. Adynamic bone disease IS REVERSIBLE, but most calcification is NOT.
  8. Measuring intact PTH (iPTH -- 1-84 PTH + 7-84 PTH) will not accurately diagnose bone turnover nor identify the majority of patients with adynamic bone disease.
  9. Measuring only a single PTH value will not accurarately diagnose bone turnover compared to measuring both 1-84 PTH and 7-84 PTH, nor will it identify the majority of patients with adynamic bone disease.
  10. Only Scantibodies Clinical Lab (SCL) measures both 1-84 PTH and 7-84 PTH, to create the AccuratioTM, which does indeed diagnose adynamic bone disease with 94% accuracy.
  11. Accurate determination of bone status allows the physician to better manage the Chronic Kidney Disease (CKD) or End-Stage Renal Disease (ESRD) patient, as well as those diagnosed with hyperparathyroidism or osteodystrophy.

Studies are on file at Scantibodies to document each point given above and also listed in the references section of this site.

SCL’s Laboratory Medical Director, Dr. Wesley Hall, is available to discuss patient test results whenever needed. Our Sales Management Team also provides highly skilled and experienced professionals in the care and testing of renal and bone disease patients. They are available to answer questions, provide materials and assist in setting up testing with SCL. (See our Sales Management Team section for contact information.)

Scantibodies Clinical Laboratory offers the physician, the dialysis center, and ultimately, the patient, a better way to diagnose and manage bone disease through better testing information, leading to the ultimate goal of better outcomes for the patient.

From Requisition to Results in 4 easy steps!

1. Complete a Test Requisition form

Note: All yellow highlighted areas are required fields and must contain all requested information. Failure to complete the requisition form in its entirety will result in a testing delay.
  1. Fill in the facility name, address and phone number.
  2. Patient name, date of birth, SSN (face sheet or patient demographic form with patient address, phone number and all billing information must be provided).
  3. Ordering doctor's name and signature of the doctor or designee. If the doctor has not tested with us before, the doctor's UPIN and Medicaid License # is required.
  4. Fill in the Medical Necessity Diagnosis (ICD-9 Code).
  5. Select the test code the doctor has requested.
  6. Fill in the date and time of the blood draw with the phlebotomist's initials.
  7. Who to bill (check one of the boxes)
    • Client
    • Patient
    • Medicare
    • Medicaid
    • Insurance (copy of insurance card - front and back) or
    • Other

Once all information is completed, submit the white and yellow copies of the requisition form with the specimen. Keep the pink copy of the form for the patient's file.

2. Prepare the Patient Sample

After extensive study, the data generated from refrigerated (not frozen) whole blood storage has demonstrated that PTH is stable compared to paired frozen plasma. Therefore, SCL is able to accept temperature monitored, refrigerated whole blood for PTH analysis in addition to frozen plasma. Please review the document, "Effect of Specimen Collection Variables on PTH Values" for more information.

Frozen Plasma

EDTA Frozen Plasma
Minimum Volume: 2ml
Temperature: -20° C (Frozen)

The specimen should be prepared in the following manner:

Using the EDTA blood collection tube:

  • Label each tube with the patient name or identifier.
  • Tap the tube gently at a point just below the stopper to release any additive adhering to the tube or stopper.
  • Permit the tube to fill completely with blood to ensure the proper ratio of blood to additive. There will be some dead space at the top of the tube.
  • To ensure adequate mixing of blood with the anticoagulant or preservative, use a slow rolling wrist motion to invert the tube gently five or six times. Failure to invert tubes may lead to the formation of microscopic clots.
  • Rapid wrist motion or vigorous shaking may contribute to hemolysis.
  • Check to see that all the preservative or anticoagulant is dissolved. If any preservative powder is visible, continue inverting the tube slowly until the powder is dissolved.
  • If multiple samples are being drawn, invert each specimen as soon as it is drawn. Do not delay inverting the samples.
  • Centrifuge the EDTA lavender top tube at 3000 rpm (8g) for 10-15 minutes.
  • Carefully remove the tube from the centrifuge to ensure the red blood cells and plasma do not re-mix.
  • After centrifugation, the plasma should be carefully removed from above the red blood cells and placed into the plastic tube that has been provided.
  • Ship the plasma tubes using dry ice so the samples remain frozen.
  • The frozen box of specimens should be shipped back to Scantibodies Clinical Lab immediately.
Refrigerated Whole Blood

EDTA Whole Blood
Minimum Volume: 6ml
Temperature: 2° C - 8° C (Refrigerated)

The specimen should be prepared in the following manner:

Using the EDTA blood collection tube:

  • Label each tube with the patient name or identifier.
  • Tap the tube gently at a point just below the stopper to release any additive adhering to the tube or stopper.
  • Permit the tube to fill completely with blood to ensure the proper ratio of blood to additive. There will be some dead space at the top of the tube.
  • To ensure adequate mixing of blood with the anticoagulant or preservative, use a slow rolling wrist motion to invert the tube gently five or six times. Failure to invert tubes may lead to the formation of microscopic clots.
  • Rapid wrist motion or vigorous shaking may contribute to hemolysis.
  • Check to see that all the preservative or anticoagulant is dissolved. If any preservative powder is visible, continue inverting the tube slowly until the powder is dissolved.
  • If multiple samples are being drawn, invert each specimen as soon as it is drawn. Do not delay inverting the samples.
  • Ship the whole blood tubes using cold packs so the samples are never frozen but remain at refrigerated temperatures.
  • The refrigerated box of specimens should be shipped back to Scantibodies Clinical Lab immediately.

3. Package and Ship the Sample to SCL

PTH samples may be transported to the laboratory either by refrigerated or frozen shipment. To ensure that the specimen integrity is not compromised, the following procedures must be followed:

How To Ship Frozen EDTA Plasma:

  • Notify SCL to arrange delivery of an approved mailing kit (see logistics number below). The kit includes: Styrofoam box, absorbent pad, dry ice, mailing tape, shipping airway bill, and the regulated sticker to ship dry ice.
  • Place the samples into the Styrofoam box with the dry ice.
  • Using the tape and shipping airway bill, prepare the box for shipment.
  • Contact Federal Express (800-463-3339) to schedule a pick up.
  • The refrigerated box of specimens should be shipped back to Scantibodies Clinical Lab immediately.

How To Ship Refrigerated Whole Blood:

  • Notify SCL to arrange delivery of an approved mailing kit (see logistics number below). The kit includes: Styrofoam box, absorbent pad, bubble wrap, gel packs, mailing tape, shipping airway bill, and an electronic temperature monitor.
  • Carefully wrap the tubes in the provided bubble wrap. Place the samples into the Styrofoam box with ice packs so the samples are never frozen but remain at refrigerated temperatures. Place the electronic temperature monitor in the shipping box with the specimens.
  • Using the tape and shipping airway bill, prepare the box for shipment.
  • Contact Federal Express (800-463-3339) to schedule a pick up.
  • The refrigerated box of specimens should be shipped back to Scantibodies Clinical Lab immediately.
Logistics & Shipping Dept.
866-249-1212 ext.524
Customer Service Dept.
866-249-1212

4. Laboratory Results

Laboratory results are available within five (5) business days of specimen receipt. A hard copy report will be mailed to the requester's office and may be faxed upon request. Our customer service department can assist you with laboratory procedures as well as any other related information.

As part of our unique service, the Medical Director of our facility is available for technical discussion or consultation on clinical results.

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